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Determination of tortoside A in Maozhiqing Oral Liquid by HPLC

Shijie Liu

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Abstract

Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid (MOL). Methods The tortoside A was determined by high performance liquid chromatography (HPLC). The Venusil MP C18 column (4.6 mm × 250 mm, 5 μm) was used, the mobile phase was acetonitrile-water (18:82), the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76 —88.00 μg/mL with an regression equation Y = 83 335X-47 442 (r = 0.999 7). The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.

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What this paper is about

Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid (MOL). Methods The tortoside A was determined by high performance liquid chromatography (HPLC). The Venusil MP C18 column (4.6 mm × 250 mm, 5 μm) was used, the mobile phase was acetonitrile-water (18:82), the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76 —88.00 μg/mL with an regression equation Y = 83 335X-47 442 (r = 0.999 7). The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.

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Available abstract

Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid (MOL). Methods The tortoside A was determined by high performance liquid chromatography (HPLC). The Venusil MP C18 column (4.6 mm × 250 mm, 5 μm) was used, the mobile phase was acetonitrile-water (18:82), the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76 —88.00 μg/mL with an regression equation Y = 83 335X-47 442 (r = 0.999 7). The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.

Key concepts: Chromatography, High-performance liquid chromatography, Reproducibility, Chemistry, Acetonitrile, Linear relationship, Analytical Chemistry (journal), Volumetric flow rate

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