Quantitative determination of 3 fat-soluble components in coronary Danshen dropping pill by HPLC

OBJECTIVE To established a method for determination of cryptotanshinone,tanshinone I,tanshinone IIA in Coronary Danshen Dropping Pills.METHODS The three fat-soluble components were extracted using ultrasonic method,Simultaneous determination of cryptotanshinone,tanshinone I and tanshinone IIA was conducted by HPLC.The column was Diamonsil~(TM) C_(18)(250 mm×(4.6 mm),5 μm);the mobile phase consisted of methanol-water(75∶25);The flow rate was(0.8) mL·min~(-1);The detection wavelength was 270 nm.RESULTS The calibration curves of cryptotanshinone,tanshinone I and tanshinone IIA were linear in the range of(1.456)-(10.19 mg)·L~(-1),(2.160)-(15.12) mg·L~(-1) and(3.152)-(22.06) mg·L~(-1) with the correlation coefficient beyond(0.9991),(0.9992) and(0.9996) respectively.The mean recoveries of the assay were(98.04%)(RSD=(1.3%),n=3) for cryptotanshinone,(99.65%)(RSD=(1.2%),n=3) for tanshinone I,and(99.31%)(RSD=(1.2%),n=3) for tanshinone IIA respectively.CONCLUSION The method is accurate and reliabile,and can be used to control the quality of Coronary Danshen Dropping Pill.