Double wavelength RP-HPLC simultaneous determination of geniposide and baicalin content in Niuhuang Shangqing tablets

Objective:To develop a double wavelength RP-HPLC method for simultaneous determination of baicalin and geniposide in Niuhuang Shangqing tablets.Method:The analysis was performed on a Hibor C18 column(150 mm×4.6 mm,5 μm)with gradient elution of acetonitrile-0.2% phosphoric acid solution.Geniposide and baicalin were detected at 240 nm and 278 nm respectively.The column temperature was 30 ℃.Results:The linear range was 0.16-0.96 μg(r=0.9999) for geniposide and 0.18-1.08 μg(r=0.9999) for baicalin.The average recoveries of geniposide and baicalin were 95.6% and 96.0% respectively,RSD were 2.7% and 2.5%.Conclusion:The method is simple,accurate and sensitive and can be used for simultaneously determining the contents of geniposide and baicalin in Niuhuang Shangqing tablets.