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Study on the expression of CD28 costimulatory molecule on human lymphocytes inhibited by special siRNA

Ying Zhang, Kailin Xu, Xiu-Ying Pan, Qun-Xian Lu

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Abstract

Different siRNA showed different reduction in CD28 expression. At 48 h post transfection, the degrees of reduction with siRNA-1, siRNA-2 and siRNA-3 were 22.10%±1.63%、73.50%±1.02% and 42.90%±0.89% respectively compared with the control (P<0.001). siRNA-2 was the most efficient. The results of semi-quantitative RT-PCR assay indicated CD28 mRNA levels were inhibited after transfection. At least 4-fold of reduction in siRNA-2 group occurred at 48 h post transfection compared with the control (P<0.001). Three different siRNA could reduce the expression of CD28 and the CD28 mRNA level. The silencing effect on CD28 mRNA induced by siRNA may contribute to costimulatory blockade. siRNA may be useful for further study on graft-versus-host disease (VGHD) after allogeneic bone marrow transplantation.

About this research paper

What this paper is about

Different siRNA showed different reduction in CD28 expression. At 48 h post transfection, the degrees of reduction with siRNA-1, siRNA-2 and siRNA-3 were 22.10%±1.63%、73.50%±1.02% and 42.90%±0.89% respectively compared with the control (P<0.001). siRNA-2 was the most efficient. The results of semi-quantitative RT-PCR assay indicated CD28 mRNA levels were inhibited after transfection. At least 4-fold of reduction in siRNA-2 group occurred at 48 h post transfection compared with the control (P<0.001). Three different siRNA could reduce the expression of CD28 and the CD28 mRNA level. The silencing effect on CD28 mRNA induced by siRNA may contribute to costimulatory blockade. siRNA may be useful for further study on graft-versus-host disease (VGHD) after allogeneic bone marrow transplantation.

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Available abstract

Different siRNA showed different reduction in CD28 expression. At 48 h post transfection, the degrees of reduction with siRNA-1, siRNA-2 and siRNA-3 were 22.10%±1.63%、73.50%±1.02% and 42.90%±0.89% respectively compared with the control (P<0.001). siRNA-2 was the most efficient. The results of semi-quantitative RT-PCR assay indicated CD28 mRNA levels were inhibited after transfection. At least 4-fold of reduction in siRNA-2 group occurred at 48 h post transfection compared with the control (P<0.001). Three different siRNA could reduce the expression of CD28 and the CD28 mRNA level. The silencing effect on CD28 mRNA induced by siRNA may contribute to costimulatory blockade. siRNA may be useful for further study on graft-versus-host disease (VGHD) after allogeneic bone marrow transplantation.

Key concepts: Transfection, Gene silencing, CD28, Small interfering RNA, Molecular biology, Transplantation, Messenger RNA, Chemistry

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