Effects of substrate ingredients on xylanase synthesis by Trichoderma reesei Rut C-30

The effects of carbon sources,nitrogen sources and ratio of carbon to nitrogen on xylanase synthesis by Trichoderma reesei Rut C-30 have been studied.The results show that the mixture of corncob xylan and sulfite pulp as a carbon soure can stimulate the synthesis of xylanase,with the increasing amount of sulfite pulp in a carbon source,xylanase activity drops after a rising.With the mixture of corncob xylan(5 g/L)and sulfite pulp(2 g/L) as a carbon source,the xylanase activity can go up to 189.24 IU/mL,with an increase of 56.66%,compared to that when corncob xylan(7 g/L)was used as the only carbon source.It's also found that the inducing ability of mixture nitrigon source is better than that of a single nitrigon source,and the inducing ability of mixture of peptone,urea and yeast extract paste is the best,which has an xylanase activity of 138.56 IU/mL.The inducing ablity of orginic nitrigon is better than that of inorginic nitrigon in single nitrigon source.The xylanase activity drops after rising with the increasing of ratio of carbon to nitrogen,the optimum ratio of carbom to nitrogen was 7.2 with corncob xylan as a carbon source.