Haemostatic Conponent of Carbonized Scutellariae Radix on Blood of Fevered and Bleeding Rats

Objective:To compare the haemostatic mechanism of carbonized and raw Scutellariae Radix,screen effective haemostatic component from the carbonized herb by fevered and bleeding rat models.Method:SD rats were randomly divided into blank control group,model compared group,Yunnan white drug group,raw Scutellariae Radix group,carbonized Scutellariae Radix group and chloroform Scutellariae Radix group.Experimental material was used to ig dose of 20 mL·g-1 once a day,for 7 days.Prothrombin time(PT),thrombin time(TT),activated partial thromboplastin time(APTT),fibrinogen content(FIB) and hemorheological parameters were examined to study the effects of experimental material on SD rats.Result:The model group had significantly difference with blank control,prolonged PT,TT,APTT,HBV,MBV and LBV to(17.26±1.73) s,(23.15±1.83) s,(22.43±1.93) s,(5.72±0.55) mPa·s,(7.44±0.78) mPa·s and(6.59±1.10) mPa·s.Carbonized Scutellariae Radix and chloroform Scutellariae Radix could significantly shorten the PT,TT and APTT of the fevered and bleeding model rats.PT,TT and APTT of carbonized Scutellariae Radix group and chloroform Scutellariae Radix group was respectively that(14.53±1.45) s,(13.24±1.46) s;(20.91±1.15) s,(18.70±1.44) s and(18.07±0.75) s,(15.59±1.74) s.Compared with the model compared group,P0.05 or P0.01.Otherwise,there was no effect on FIB.Conclusion:The chloroform extract is the effective component for hemostasis,and plays roles by ways of endogenous thrombin,extrinsic thrombin and the both,but has no relation with fibrin.The improvement of hemorheological parameters is a basis for hemostasis.