Studies on the genetic variation of 9 populations of Tricula by SSR-PCR

Objective To study the genetic variation of 9 populations of Tricula and show the evidence for the classification of Tricula on genetic level. Methods Tricula isolates were obtained from seven areas in China.Their genomic DNA was amplified by the simple sequence repeat-anchored PCR(SSR-PCR) technique.Their genetic distances were measured according to their amplified products and the corresponding dendrogram was constructed. Results The SSR-PCR products were polymorphic.The genetic distance between the Chinese mainland isolates of Tricula and the Taiwan isolate was 0.7391-0.9130;The similarity between Tricula hsiangi and Tricula pingi was sufficiently close,and the genetic distance was 0.2174;The genetic distance between Shennongjia and Wufeng(T.pingi and T.hsiangi) isolates was 0.3333-0.3913;The genetic distance between Shiyan and Zhongxiang isolates was 0.5556;The genetic distance between Fujian and Hubei isolates was 0.3913-0.7000;The genetic distance between Guangxi and Hubei isolates was 0.6667-0.8889.Conclusion The population of Tricula can be divided into four groups in gene level: 1)isolates from Wufeng(T.pingi and T.hsiangi) and Shengnongjia;2)isolates from Zhongxiang(T.hongshanensis) and Shiyan;3)isolates from Wuming;4)isolates from Fujian(T.fujianensis).And at genetic level,the evidence Tricula chiui was a kind of Ocomelania was proved.