Effect of Xingnao Kaiqiao Acupuncture on Expression of ICAM-1 and P-selectin after Cerebral Ischemia and Reperfusion in Rat

Objective To explore the mechanism of Xiangnao kaiqiaorepreshment and Inducing resuscitation acupuncture against cerebral ischemia and reperfusion injury. Methods The cerebral ischemia and reperfusion injury was established by lineembolism of cerebral middle artery. The expression of ICAM-1 and P-selectin mRNA and protein in ischemic cerebral cortex and corpus striatum in rat was observed after 1 h of cerebral ischemia and 361224 and 48 h of reperfusion by in situ hybridizatioin and immunohistochemistry. Results The mRNA expression of ICAM-1 and P-selectin was found at the capillary endothelial cells in the postischemia cerebral cortex 3 h after reperfusion and peaked at 12 h and 6～12 h respectivelyP0.01 and increased continually during 48 reperfusion. Both expressions of mRNA and protein of ICAM and P-selectin in ischemia cerebral cortex were down-regulated considerably by Xiangnao Kaiqiao acupunctureP0.01. Conclusion The Xingnao Kaiqiao acupuncture method may prevent and cure the cerebral ischemia and reperfusion injury by down-regulating the expression of mRNA and protein of ICAM -1 and P-selectin in ischemia cerebral cortex.