Effects of bone marrow stromal cell transplantation on hypoxic ischemic brain damage in neonatal rats

Aim:To observe/effects of bone marrow stromal cells(BMSCs) transplantation on hypoxic ischemic brain damage(HIBD) in neonatal rats.Methods:Thirty 7-day-old SD rats were randomly divided into 3 groups:sham group(n=6),HIBD group(n=12),and transplant ation group(n=12).BMSC stransfected GFP with were transplantated into ischemic coxtex of model neonatal rats of transplan tation group.After transplantation for 14 d,the expression percentage of NeuN,NF,MAP-2,GFAP and S-100 were detected by immunocytochemical technique in the GFP positive cells.NSS were assessed.Results:The hippocampal neurous remained alive well and the cellular structuve remained intact sham group while hippocampal stracture were destructed and only a few cells were alwe HIBD group.In transplantation group,the orgamzation structure was almost intact,and GFP positive cells there were a lot of in transplantation zone,and in the GFP positive cells the expressive percentage of marked protein of neurons and glial cells were detected:NeuN were(5.4±1.4)%,NF were(3.3±1.6)%,MAP-2 were(4.4±2.4)%,GFAP were(14.2±4.1)%,S-100 were(15.4±4.5)%.The NSS scale in sham group,HIBD group and transplantation group were(7.9±1.3),and(5.1±1.1),there were significant differences(F=62.57,P0.05).Conclusion:BMSCs transplantation,into brain can reduce the injurg induced by HIBD in neonatal rats.