Analysis on Components of HMW-GS in New Wheat Cultivars(Lines) in Huanghuai Wheat Zone

High molecular weight subunit(HMW-GS) components of 113 new wheat(Triticum aestivum L.) cultivars(lines) were fractionated by SDS-PAGE in Huanghuai wheat zone.12 HMW-GS alleles were identified.Subunits 1,2* and null at Glu-A 1 were found with frequencies of 68.14%,4.42% and 27.43%,respectively;subunits 7+8,7+9,14+15,13+16,13+19 and 17+18 at Glu-B 1 with frequencies of 37.17%,49.56%,10.62%,0.88%,0.88% and 0.88%,respectively;and subunits 2+12,4+12,5+10 at Glu-D 1 with frequencies of 43.36%,33.63% and 23.01%,respectively.There were 23 types of subunits or combination of subunits.Among them were some fine subunits or combinations of subunit,which could promote the quality of wheat-breeding,such as 5+10,1,2*,7+8,14+15,17+18,and 13+16.However,the frequencies of fine subunits for 5+10,2* were still low.It was proposed that more excellent parents for wheat-breeding should be introduced and exploited in the future.