2006•Journal of Nantong UniversityRequires access
Protective effects of never regeneration factor on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells
Min Zheng
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Abstract
Objective:To study the protective effects of NRF on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells.Methods:With the hypoxia/reoxygenation injury model of cultured neonatal rat myocardial cell,the modality of the cells and the levels of the SOD,MDA,LDH,dehydrogenase in mitochondria were detected to study the effects of NRF on hypoxia/reoxygenation injury.Results:Refracting power of the cells declined and the beating became weak or even stopped in H/R group.Compared with the control group,the activities of SOD and dehydrogenase in mitochondria decreased(P0.01)while the contents of LDH and MDA increased.Compared with H/R group,the activities of SOD and dehydrogenase in mitochondria increased(P0.01) and the contents of LDH and MDA decreased significantly in each NRF group(P0.01).Conclusion:NRF has protective effects on the cultured neonatal rat myocardial cells injured by hypoxia/reoxygenation.The mechanisms are related to its inhibition of lipid peroxidation.