Experimental study on protective effect of ischemic preconditioning on renal cold ischemia reperfusion injury in rat

Objective To investigate the protective effect of ischemic preconditioning on rat with renal cold ischemia reperfusion injury.[WTHZ]Methods Thirty-six SD rats with cold ischemia reperfusion injury were randomly divided into the following 3 groups: the sham operated group(A),cold ischemia reperfusion group(B),and treatment group(C) receiving ischemic(5 minutes) and reperfusion(5 minutes) three times.The expression of Bcl-2 and Bax protein were observed with immunohistochemistry.The level of renal function,SOD,MDA and histologic examination were detected.Results Blood urea nitrogen and creatinine levels in group B and C were significantly higher than in group A(P0.01),group C was lower than group B(P0.01).SOD in group C was significantly higher than in group B(P 0.01),but MDA decreased significantly(P(0.01)).Bcl-2 and Bax were all increased in group B and group C as compared with that of group A(P0.01).Bcl-2 was increased but Bax was decreased significantly in group C than in group B(P0.01).Group C showed a less pathological change and a lighter renal impairment(P0.01).Conclusion Ischemic preconditioning has protective effect on renal cold ischemia reperfusion injury through regulating Bcl-2 and Bax expression.