Research on optimization of experiment conditions for determination of 1,3-diphenyl-1,3-propanedione in mouse brain with orthogonal experiment design

Objective:To optimize the experiment conditions for the determination of 1,3-diphenyl-1,3-propanedione in mouse brain with orthogonal experiment design and to improve the sensitivity of the measurement method. Methods:The best extraction agent was selected by group contribution method and then the experiment conditions were optimized with orthogonal experiment design. Results:The best extraction agent for 1,3-diphenyl-1,3-propanedione in mouse brain was ethyl acetate/Triton X-100(90:10,v/v),and the extraction efficiency was the highest when the extraction agent volume was 400 μl,the ratio of Triton X-100 was 20%,the extraction time was 3 min,the centrifugation time was 4 min and the ratio of water in constant volume solution was 10%. The lower quantitative limit of DPPD was 2 μg/ml,the recovery were 103. 5% ~ 112. 0%,and most of relative standard deviation of intra-day and inter-day precision were all less than 10% under the optimized conditions. Conclusion:The optimization results was satisfactory when applying orthogonal experiment design to optimize the experiment conditions for the determination of 1,3-diphenyl-1,3-propanedione in mouse brain. The method can satisfy the needs of actual test.