Effect of sCD40L on the secretion of tumor necrosis factor-α in human umbilical vein endothelial cells

Objective To investigate the effect of sCD40L on the secretion of tumor necrosis factor-α( TNF-α)in human umbilical vein endothelial cells( HUVEC).Methods The HUVECs were cultured in different groups,and HUVECs were stimulated by r-CD40L at different concentrations( 0,10,20,30,40 μg/L)and in different time points( 0,10,20,30,40 min),then the concentrations of TNF-α in supernatant were detected by enzyme-linked immunosorbent assay( ELISA).Results The levels of TNF-α secreted by HUVEC in supernatant were the highest at 20 minutes after stimulation by r-CD40L( 20 μg/L),there were significant differences among the 5 different time groups( P 0.01).With the increase of concentration of r-CD40L,the levels TNF-α secreted by HUVEC in supernatant were increased in a dose-dependent manner,there were significant differences among the 5 different dose groups( P 0.01).Conclusion The levels of TNF-α secreted by HUVEC may reach peak at early time after r-CD40L stimulation,moreover,which is in a concentration-dependent manner.