Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of Lonicera japonica with Polyamide Resin

Objective:To optimize separation and purification technology of chlorogenic acid in leaves of Lonicera japonica.Method:With the content of chlorogenic acid as index,static and dynamic absorption,desorption properties of polyamide resin were observed by single factor test,and sample amount,the amount of washing water,type and dosage of eluent were investigated;With purity and retention rate as index,effects of elution flow rate,the concentration and volume of eluent on separation and purification technology was investigated by orthogonal test.Result:Optimized separation and purification technology was as following:sample amount 10 mg·g-1 resin,washed impurity with 2 BV water,and eluated with 3 BV the amount of 65% ethanol,flow rate of 0.4 BV·h-1,collected eluent.Conclusion:This optimized technology was stable and practicable,it could provide reference for industrial separation and purification of chlorogenic acid.