Hypoglycemic Function of Polysaccharides from Brassica rapa L.of Xinjiang

【Objective】To investigate the composition and structure of polysaccharides from Brassica rapa L.of Xinjiang and its effects on blood sugar level.【Method】Crude polysaccharide was extracted from Brassica rapa L.by common water extraction method,and then deproteinized by Sevag method.The crude polysaccharide from Brassica rapa L.was further purified by refluxing and dialyzing,and then purified polysaccharide was obtained.Blood sugar level test was performed on white mice by using purified polysaccharide.DEAE-52 ion-exchange column chromatography was performed on purified polysaccharide and Sephadex G-200 gel column chromatography was performed on the first component BRPS1 for purity analysis.And then infrared analysis was performed on BRPS1;seven components obtained by ion-exchange column chromatography were analyzed by GC-MS for determining its monosaccharide composition.【Result】Under the dose of 400 mg/kg bw: after 3 days the blood sugar level decreased relatively obviously(P0.05); after 6 days and 9 days the blood sugar level decreased quite obviously(P0.01 and P0.001 respectively).Seven components were derived by DEAE-52 ion-exchange column chromatography.Result of Sephadex G-200 gel column chromatography showed that BRPS1 was pure polysaccharide.Infrared analysis indicated that BRPS1 had common composition of polysaccharides.Six kinds of monosaccharides were identified in Brassica rapa L. purified polysaccharides by GC-MS.【Conclusion】Polysaccharides from Brassica rapa L.of Xinjiang has obvious hypoglycemic effect;BRPS1,as the main component of Brassica rapa L.polysaccharides,is pure polysaccharide,and it has the common structure of polysaccharides.