RAPD Analysis on Genetic Variation of Bambusa pervariabilis McClure

Thirty clumps of six populations of Bambusa pervariabilis were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variations among and within the populations. A total of 173 loci including 85 polymorphic loci were amplified using 28 random primers, with an average of 6.18 fragments each primer. The length of fragments was between 200 bp and 2 000 bp. As analyzed by POPGENE version 1.31, the data from six populations had an average Nei's gene diversity of 0.211 4, and Shannon's genetic diversity of 0.327 7, coefficient of gene differentiation(Gst) of 0.185 3, indicating that there was some differentiation among the populations. The average genetic distance among populations was 0.035 0, indicating that there was close relative relations among populations. The six populations were clustered to three categories by cluster analysis (UPGMA) based on Nei's unbiased genetic distance.