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A Study on Tissue Culture of Phalaenopsis spp.

Lifang Huang

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Abstract

The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L+NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L+NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L + NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L+NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy for protocorm-like body differentiation to buds in 1/2MS+6-BA1.0mg/L+NAA0.5mg/L. In rooting stage, the 1/2MS medium with IBA0.3 mg/L was efficient. Transplanted them on water moor, and its survive rate reached 100% after two months. In addition, it was more conducive to the growth by water culture in the late cultivation.

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What this paper is about

The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L+NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L+NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L + NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L+NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy for protocorm-like body differentiation to buds in 1/2MS+6-BA1.0mg/L+NAA0.5mg/L. In rooting stage, the 1/2MS medium with IBA0.3 mg/L was efficient. Transplanted them on water moor, and its survive rate reached 100% after two months. In addition, it was more conducive to the growth by water culture in the late cultivation.

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Available abstract

The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L+NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L+NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L + NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L+NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy for protocorm-like body differentiation to buds in 1/2MS+6-BA1.0mg/L+NAA0.5mg/L. In rooting stage, the 1/2MS medium with IBA0.3 mg/L was efficient. Transplanted them on water moor, and its survive rate reached 100% after two months. In addition, it was more conducive to the growth by water culture in the late cultivation.

Key concepts: Phalaenopsis, Shoot, Tissue culture, Biology, Botany, Horticulture, In vitro, Biochemistry

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