Induction,preservation and differentiation of calliof Pyrethrum cinerariifolium Trev.

With stem tip as explants and MS as basic culture medium,studies were carried out on continuing growth and induction of subculture of Pyrethrum cinerariifolium Trev..The results show that:among the nine different treatments with the combinations of 2,4-D and KT,the better one is 1.0 mg/L vs 0.4 g/L.By doing so,we can get an ideal induction rate 88.0%;when the concentration of sucrose is ranged from 10 to 40 g/L,it will be in the direct proportion with increment of callus,while with the inverse proportion with start time of callus browned.Especially when the concentration of sucrose is 25 g/L,the callus increment can get to 0.127 g/d;at the same time,proper illumination can prolong the preserving time and maintain an upper tissue structure.If it being illuminated by 4 h/d,the beginning time of being browned of callus will exceed 27 days.