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2010•The Chinese Journal of Modern Applied PharmacyRequires access

Study on Quality Standard of Tangzu Granule

Feng Ying

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Abstract

OBJECTIVE To establish the quality standard for Tangzu granule.METHODS Thin-layer chromatography(TLC) was employed to identify Radix Astragali,Radix Stephaniae tetrandrae and Radix Angelicae Sinensis,while high performance liquid chromatography(HPLC) was applied for the determination of paeoniflorin in Radix Paeoniae Rubra in Tangzu granule.RESULTS The identification by TLC was distinct and highly specific.A linearity was obtained from 0.504 μg to 5.040 μg of paeoniflorin in Tangzu granule with a good correlation(r=0.999 8,n=6).The average recovery was 97.74%,and RSD was 0.72%(n=6).CONCLUSION The method is reliable,accurate and specific and can be used for quality control of Tangzu granule.

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OBJECTIVE To establish the quality standard for Tangzu granule.METHODS Thin-layer chromatography(TLC) was employed to identify Radix Astragali,Radix Stephaniae tetrandrae and Radix Angelicae Sinensis,while high performance liquid chromatography(HPLC) was applied for the determination of paeoniflorin in Radix Paeoniae Rubra in Tangzu granule.RESULTS The identification by TLC was distinct and highly specific.A linearity was obtained from 0.504 μg to 5.040 μg of paeoniflorin in Tangzu granule with a good correlation(r=0.999 8,n=6).The average recovery was 97.74%,and RSD was 0.72%(n=6).CONCLUSION The method is reliable,accurate and specific and can be used for quality control of Tangzu granule.

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Available abstract

OBJECTIVE To establish the quality standard for Tangzu granule.METHODS Thin-layer chromatography(TLC) was employed to identify Radix Astragali,Radix Stephaniae tetrandrae and Radix Angelicae Sinensis,while high performance liquid chromatography(HPLC) was applied for the determination of paeoniflorin in Radix Paeoniae Rubra in Tangzu granule.RESULTS The identification by TLC was distinct and highly specific.A linearity was obtained from 0.504 μg to 5.040 μg of paeoniflorin in Tangzu granule with a good correlation(r=0.999 8,n=6).The average recovery was 97.74%,and RSD was 0.72%(n=6).CONCLUSION The method is reliable,accurate and specific and can be used for quality control of Tangzu granule.

Key concepts: Paeoniflorin, Granule (geology), Quality standard, Chromatography, High-performance liquid chromatography, Chemistry, Thin-layer chromatography, Materials science

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