ScholarLens Back to search
2011•Journal of Food Science and BiotechnologyRequires access

Cloning and Sequence Analysis of β-1,3-1,4-Glucanase Gene

Hong Yao

Open publisher page 0 citations

Abstract

Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase(bgl) was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines(M15674),B.subtilis(D00518) and B.licheniformis(AY365256)were 99%,95% and 94%,respectively.

About this research paper

What this paper is about

Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase(bgl) was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines(M15674),B.subtilis(D00518) and B.licheniformis(AY365256)were 99%,95% and 94%,respectively.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase(bgl) was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines(M15674),B.subtilis(D00518) and B.licheniformis(AY365256)were 99%,95% and 94%,respectively.

Key concepts: Bacillus amyloliquefaciens, Glucanase, Biology, Gene, Restriction enzyme, Sequence analysis, Plasmid, Cloning (programming)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Sequence Analysis of β-1,3-1,4-Glucanase Gene — Research Paper | ScholarLens