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Cloning and Sequencing of Endo-Glucanase Gene of Bacillus.megaterium AP25

Yang He-tong

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Abstract

Bacillus.megaterium AP25 screened from Australia wet soil could produce endo-β-1,4-glucanase that was detected on CMC-Na plates.We amplified the endo-β-1,4-glucanase gene and sequenced it according to GenBank No.DQ782954.1,M28332.1,AY859492.1.Its full length was 1500bp and its amino acid number was about 499.Its amino acid sequence had 98% homology with that of the endo-β-1,4-glucanase gene of Bacillus subtilis.

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What this paper is about

Bacillus.megaterium AP25 screened from Australia wet soil could produce endo-β-1,4-glucanase that was detected on CMC-Na plates.We amplified the endo-β-1,4-glucanase gene and sequenced it according to GenBank No.DQ782954.1,M28332.1,AY859492.1.Its full length was 1500bp and its amino acid number was about 499.Its amino acid sequence had 98% homology with that of the endo-β-1,4-glucanase gene of Bacillus subtilis.

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Available abstract

Bacillus.megaterium AP25 screened from Australia wet soil could produce endo-β-1,4-glucanase that was detected on CMC-Na plates.We amplified the endo-β-1,4-glucanase gene and sequenced it according to GenBank No.DQ782954.1,M28332.1,AY859492.1.Its full length was 1500bp and its amino acid number was about 499.Its amino acid sequence had 98% homology with that of the endo-β-1,4-glucanase gene of Bacillus subtilis.

Key concepts: Bacillus megaterium, Glucanase, Bacillus subtilis, GenBank, Homology (biology), Gene, Cloning (programming), Bacillaceae

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