Determination of Free,Total and Binding Anthraquinones in Liuwei Anxiao Capsules with HPLC

Objective: To develop an HPLC method for determination of free anthraquinone,total anthraquinone and binding anthraquinone in Liuwei Anxiao capsules.Methods: Hypersil C18 column(200 mm×5.0 mm,5 μm) was adopted.The mobile phase was methnol-0.1% phosphoric acid(80∶20).The flow rate was 1 mL·min-1 and the detection wavelength was 254 nm.Results: The linear ranges of emodin and chrysophanol were 0.016 2～0.323 2μg and 0.0356～0.7128μg,respectively.The average recoveries were 100.08%(RSD=0.37%) for emodin,and 99.11%(RSD=2.70%) for chrysophanol in free anthraquinone,and 101.62%(RSD=2.40%) for emodin,and 100.54%(RSD=1.37%) for chrysophanol in total anthraquinone,respectively.Conclusion: The method is convenient,accurate and can be used for determination of the preparation.