Effect of Cytokines (Interleukin-6) on Interleikin-1 Receptors in the Hippocampus of Rats Following Global Brain Ischemia-reperfusion

Objective: In order to explore effect of interleukin-6 (IL-6), which was regarded as a potential endogenous neuroprotective cytokine against brain ischemia-reperfusion injury, investigate the relationship between it and another interleikin (IL-1) and provide a kind of experimental and theoretic basis. Methods:After the 26 Wistar rats were divided into two groups, the control group(n = 9) and the experiment group(n = 17), their lateral ventricles were injected into the same amount of Saline and IL-6 respectively. Then they were formed into the global brain ischemia/reperfusion models by the quadri-arteries block pattern, and the IL-1R immunoreactivity of their CA1 and CA3 areas in hippocampus were observed and assayed statistically. Results: 4 h after the global brain ischemia reperfusion the IL-1R immunoreactivity of the CA1 and CA3 areas in hippocampus increased significantly, from which we can discover the more immunoreactivity positive cells and their more deeper staining. Conclusions: IL-6, as a neuroproteceive factor in the pathogenesis of the brain ischemia-reperfusion injury of the rats, may fulfill its neuroprotective function according to inhibiting the receptor of the IL-1 in CNS.