Separation, Purification and Identification of Lauroyl Maltoses

The lauroyl maltoses synthesized by lipase were separated and purified by the thin layer chromatography (TLC) and silica gel column chromatography, and then the purified products were analyzed by HPLC, MS, IR and NMR. The optimum conditions for TLC were confirmed: 5μl of the reaction mixture were applied to silica gel G plates, developed by chloroform- methanol (4:1, V/V), and then detected by spraying with 5% of sulfuric acid in ethanol and heated at 120 ℃ for 20 min. The optimum conditions for silica gel column chromatography were: 10 ml of the reaction mixture was applied to 12 mm×1000 mm silica gel column (100～200 mesh) and chloroform-methanol (4:1, V/V) was used as mobile phase, whose flow rate was 18 ml/h, the eluent of 1 tube /10 min was collected. The purified products were 6'-O- lauroyl maltoses and 6, 6'-O- lauroyl maltoses.