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A comparative study of HPLC and TLC separation of amino acids using Cu(II) ion

Ravi Bhushan, Imran Ali, Shalini Sharma

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Abstract

Separation of amino acids using Cu(II) by RP-HPLC and impregnated TLC is reported. For HPLC the sample mixture of amino acids containing copper (II) was injected into the column while for TLC the silica gel plates were impregnated with Cu(II). The mobile phase used in HPLC was acetate buffer (0.3 M, pH 6.0)-acetonitrile (9:1, v/v), and that in TLC was acetate buffer (0.3 M, pH 6.0):acetonitrile-n-butanol (12:5:10, by vol.). The results have been compared and discussed.

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What this paper is about

Separation of amino acids using Cu(II) by RP-HPLC and impregnated TLC is reported. For HPLC the sample mixture of amino acids containing copper (II) was injected into the column while for TLC the silica gel plates were impregnated with Cu(II). The mobile phase used in HPLC was acetate buffer (0.3 M, pH 6.0)-acetonitrile (9:1, v/v), and that in TLC was acetate buffer (0.3 M, pH 6.0):acetonitrile-n-butanol (12:5:10, by vol.). The results have been compared and discussed.

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OpenAlex reports 11 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Separation of amino acids using Cu(II) by RP-HPLC and impregnated TLC is reported. For HPLC the sample mixture of amino acids containing copper (II) was injected into the column while for TLC the silica gel plates were impregnated with Cu(II). The mobile phase used in HPLC was acetate buffer (0.3 M, pH 6.0)-acetonitrile (9:1, v/v), and that in TLC was acetate buffer (0.3 M, pH 6.0):acetonitrile-n-butanol (12:5:10, by vol.). The results have been compared and discussed.

Key concepts: Chemistry, Chromatography, Acetonitrile, High-performance liquid chromatography, Silica gel, Copper, Amino acid, Organic chemistry

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