Effects of Chinese medicine YiBiQing on bacteria translocation in acute necrotizing pancreatitis in rabbits

Objective To investigate the effects of Chinese medicine YiBiQing on bacteria translocation in acute nectrotizing pancreatitis (ANP) in rabbits. Methods Retrograde pancreatic duct injection of 5% sodium taurocholate to establish ANP models. All rabbits were randomly divided into three groups:the sham-operative group(group A, n = 15), the ANP group(group B, n =15), and the ANP with Chinese medicine group(group C, n = 15) . The changes of serum/plasma, endotoxin, and motilin( MTL), the level of serum TNF-a 24 h postoperation, and the bacterial translocation from intestinal lumen were examined. Results At the 3rd and 7th day postoperation, the levels of plasma endotoxin in group C were significantly lower than those in group B [plasma endotoxin, 3rd day: (112 ± 14) Eu/ml vs(321±14) Eu/ml ( P 0.01) ;7th day: (105 ± 9) Eu/ml vs (178 ±32) Eu/ml ( P 0.05)]; At the first day postoperation, the levels of plasma MTL in group B was lower than that in preoperation, but in group C, the results were adverse, At the first, 3rd and 7th day, the levels of plasma MTL in group C was higher than those in group B ( P 0.01) ; the level of serum TNF-a 24 h postoperation in group C was lower than that in group B [(1.87±0.22)μg/L vs (2.83±0.31)μg/L, P 0.05];the positive rate of bacteria cuture with mesenteric lympha node and pancreas, lung in group C was significantly lower than that in group B (4/14 vs 9/9, P 0.01 and 3/14 vs 8/9,2/14 vs 7/9, P 0.05).Conclusion The Chinese medicine YiBiQing is able to accelerate the motion of gastrointestine and output the contents of intestinal lumen, protect the barrier of intestinal mucosa, and reduce the bacteria translocation from intestinal lumen.