Study of combined hyperbaric oxygen and ulinastatin treatment on acute necrotizing pancreatitis in rats

Objective To study the combined hyperbaric oxygen(HBO) and ulinastatin treatment on plasma endotoxin,sCD14and plasma endotoxin inactivation capacity(EIC) in acute necrotizing pancreatitis(ANP) in rats,and its effect on prognosis.Methods SD rats were divided into control group(group A),sham operation group(group B),ANP group(group C),ANP+HBO group(group D),ANP+unlinastatin group(group E),and ANP+HBO+unlinastatin group(group F) randomly.Rat ANP model was made by retrograde injection of 3.5% sodium taurocholate(2.5 ml/kg) via the pancreatic duct;5 min after model had been made,group E and group F were injected with unlinastatin(20 000 U/kg) via portal vein;30 mins after model had been made,group D and group F were given hyperbaric oxygen treatment for 2h,3h,6h and 10h,after which,the plasma endotoxin,sCD14,EIC,TNFa and survival time in each group were examined individually.Results(1) Endotoxin and sCD14: At 3h and 6h after model had been made,the levels of plasma endotoxin and sCD14 in group D and group E were significantly lower than those in group C(P0.05).At 3h,6h and 10h after model had been made,the levels of plasma endotoxin and sCD14 in group F were significantly lower than those in group C,group D and group E(P0.05).(2) EIC: At 3h,6h and 10h after model had been made,the levels of EIC in group D,group E and group F were significantly higher than those in group C,group D and group E(P0.05).the level of EIC in group F was significantly higher than that in group C and group D(P0.05).(3) TNF-α: At 3h,6h and 10h after model had been made,the level of TNF-α in group D,group E and group F was significantly lower than that in group C(P0.05).the level of EIC in group F was significantly lower than that in group D and group E(P0.05).(4)Survival time: the survival time in group D,group E and group F was significantly longer than those in group C(P0.05).the survival time in group F was longer than that in group D and group E significantly(P0.05).Conclusions Hyperbaric oxygen or ulinastatin is individually effective in ANP,and combined hyperbaric oxygen and ulinastatin treatment is much more effective than either of them.