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[Study on enzyme-solvent extraction process of flavonoids from leaves of Ginkgo biloba].

Ouyang Na-na, Xiangzhou Li, Zhengyuan Luo

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Abstract

OBJECTIVE: To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba. METHODS: To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids. RESULTS: The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%. CONCLUSION: The method is simple and can increase material utility effectively.

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What this paper is about

OBJECTIVE: To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba. METHODS: To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids. RESULTS: The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%. CONCLUSION: The method is simple and can increase material utility effectively.

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Available abstract

OBJECTIVE: To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba. METHODS: To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids. RESULTS: The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%. CONCLUSION: The method is simple and can increase material utility effectively.

Key concepts: Ginkgo biloba, Ginkgo, Extraction (chemistry), Chemistry, Yield (engineering), Chromatography, Solvent, Enzymatic hydrolysis

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