Fine mapping of awn gene in Sorghum bicolor( L.) Moench

The inheritance character of the awnness of sorghum were investigated in F1and 1 155 individuals of F2segregation population derived from a cross between awnless cultivar Btx623 and awned cultivar 7361. It was showed that the trait of awnness was controlled by a single recessive gene. The polymorphism of totally 250 SSR markers distributed throughout the genome of sorghum were identified. The polymorphic markers and 22 recessive F2plants were used to primarily map the gene. The gene was located between markers sm03100 and sm03108 on chromosome 3,with genetic distances of 11. 4 cM and 4. 5 cM,respectively. To narrow down the located interval,InDel markers were developed through sequencing 7361 and alignment with Btx623,and the mapping population was enlarged to 214 recessive F2plants. With newly mined InDel markers and enlarged population,the target region harboring Awn3. 1 was finally narrowed down to a 115 kb fragment between markers InDel-1 and InDel-2.