2010•Zhongguo Nongye Daxue xuebaoRequires access
Mapping awn gene AWN3-1 with near-isogenic line of rice
Yao Guo-xin, Qiang Zhang, Wu JianTao, Guanglong Hu, Zichao Li
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Abstract
Long-awn trait was preserved from wild rice(Oryza rufipogon),but in modern,the awnless varieties were developed by breeder.Up to now,there was no report about fine mapping or cloning awn gene.We developed a backcross between long-awn SLG(Japonica) as donor came from National Germplasm Library and awnless Nipponbare(Japonica),and selected a small BC4F2 population which segregation ratio of awn accorded with 3∶1 to develop a large mapping population with heterozygous BC4F2 plants.Using bulked segregant analysis(BSA),from 1 512 SSR makers evenly distributed on rice genome,we rough mapped the awn gene between RM6283 and RM5685 on chromosome 3,named AWN3-1.After developed and screened new markers,AWN3-1 was fine-mapped between marker Y5 and marker Y9,which genetic distance was 0.5 and 0.4 cM,respectively.This work provided a useful help for cloning AWN3-1.