Cloning and Sequence Analysis of Chinese Cabbage Chitinase Gene CHB4

Based on the conserved sequence of the chitinase of crucifers,about 1 kb DNA fragment and 5'-end fragment(150 bp)were amplified by PCR from genomic DNA of Chinese cabbage.Experiments were performed on 7-day-old Chinese cabbage seedlings after germination with salicylic acid of different concentrations.Total RNA was prepared from the plant with higher chitinase activity,and 3'-end fragment was amplified by 3' RACE.Comparing Chinese cabbage chitinase sequence cloned in this experiment with that reported in GenBank composed of 1 094 bp nucleotide of Brassica napus,the experiment showed that the chitinase sequence of Chinese cabbage is 1 517 bp in size with one intron of 508 bp(DDBJ accession number:AB257452),and shares as high as 99% identity with B.napus and over 50% with other high plants in coding region.