Detection of urinary vascular endothelial growth factor and its clinical significance in type 2 diabetes mellitus

Objective To study the changes of urinary vascular endothelial growth factor(UVEGF) excretion and its clinical significance in type 2 diabetes mellitus(T2DM) patients with different urinary albumin excretion rate.Method For 22 normal controls and 65 of type 2 diabetics were recruited into this study.The 65 type 2 diabetics were divided into 3 groups according to urinary albumin excretion rate(UAER):normal albuminuria group(n =23),microalbuminuria group(n =22),clinical albuminuria group(n =20).Urinary VEGF levels were measured by using ELISA method.Results ①UVEGF excretion rate in normal albuminuria group was obviously higher than that in controls(P 0.05).In T2DM group,UVEGF excretion rate in microalbuminuria and clinical albuminuria groups were significantly higher than that in normal albuminuria group(P 0.05 or P 0.01).UVEGF excretion rate in clinical albuminuria group was obviously higher than that in microalbuminuria group(P 0.05).There was significant differences among each group.②UVEGF excretion rate was positively related to UAER,plasma creatinine and course of diseases.③Multiple regression analysis displayed that UAER and plasma creatinine were affecting factors of UVEGF excretion rate.Conclusion Increases of UVEGF in type 2 diabetes are ahead of changes of UAER and are related with the degree of pathological changes of kidney.UVEGF might be a sensitive factor of the early stage of diabetic nephropathy.It might predict the evolvement of diabetic nephropathy.