Isolation and Identification of Porcine Parvovirus

One local strain of porcine parvovirus was isolated from the dead fetus in Fujian province.The isolated virus could cause typical cytopathogenic effect(CPE) in PK-15cell at hour 36after infection such symptoms as shrunk,aggregated,and detached.The isolated virus could be neutralized by the positive sera.The DNA extracted from the virus isolate was subjected to polymerase chain reaction(PCR) analysis,a set of primer which can be used to amplify 531bp fragment in the gene of PPV was designed.The homology of VP2gene sequence between the isolated viruses and NADL-2from GenBank was 99.2%,it was indicated that the isolated virus was porcine parvovirus(PPV).In conclusion,this study laid the foundction for further development of the pathogenic mechanism of virus,epidemiology,diagnostic studies and vaccine.