Construction and Identification of Eukaryotic Expression Vector of SDF-1α Gene

Objective To construct an eukaryotic expression vector of SDF-1α gene and to provide a tool for studying its function.Methods The cDNA of SDF-1α was amplified by RT-PCR and PCR.After purification,the gene was cloned into eukaryotic expression vector pcDNA3.1.The constructed recombinant plasmid was identified by PCR confirmation,enzyme digestion and DNA sequencing. Results The constructed recombinant plasmid pcDNA3.1/SDF-1α was obtained,positive clones were screened and identified by PCR and digestion with restriction enzyme.The sequence of SDF-1α was in accordance with the expected one.Conclusion The eukaryotic expression vector of SDF-1α gene has been successfully constructed,which may provide a basis for further researches.