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2015•Guizhou nongye kexueRequires access

Optimization of Solid-state Fermentation Condition of Aspergillus niger Strain with Producing Cellulase

Zhou Chenya

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Abstract

The effect of different carbon source,nitrogen source,surfactant,initial pH,medium material/water ratio,temperature and time based on main raw materials of wheat bran and corn cob on cellucase activity of A.niger XZ-3Sstrain by single-factor and L9(34)orthogonal test and then the solidstate fermentation condition of A.niger XZ-3Sstrain with producing cellulase was optimized to improve utilization efficiency of cellulose.Results:The optimal fermentation condition of A.niger XZ-3Sstrain with producing cellulase includes 6∶2of wheat bran/corn cob ratio,4.0% ammonium sulfate,0.4%Twain-40,initial pH 4.0,28℃,96 hand 1∶1.5of medium material/water ratio.The activity of CMC and FPA enzyme of A.niger XZ-3Sstrain with producing cellulase can reach 273.56IU/g and 135.61IU/g under the optimal fermentation condition respectively.

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The effect of different carbon source,nitrogen source,surfactant,initial pH,medium material/water ratio,temperature and time based on main raw materials of wheat bran and corn cob on cellucase activity of A.niger XZ-3Sstrain by single-factor and L9(34)orthogonal test and then the solidstate fermentation condition of A.niger XZ-3Sstrain with producing cellulase was optimized to improve utilization efficiency of cellulose.Results:The optimal fermentation condition of A.niger XZ-3Sstrain with producing cellulase includes 6∶2of wheat bran/corn cob ratio,4.0% ammonium sulfate,0.4%Twain-40,initial pH 4.0,28℃,96 hand 1∶1.5of medium material/water ratio.The activity of CMC and FPA enzyme of A.niger XZ-3Sstrain with producing cellulase can reach 273.56IU/g and 135.61IU/g under the optimal fermentation condition respectively.

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Available abstract

The effect of different carbon source,nitrogen source,surfactant,initial pH,medium material/water ratio,temperature and time based on main raw materials of wheat bran and corn cob on cellucase activity of A.niger XZ-3Sstrain by single-factor and L9(34)orthogonal test and then the solidstate fermentation condition of A.niger XZ-3Sstrain with producing cellulase was optimized to improve utilization efficiency of cellulose.Results:The optimal fermentation condition of A.niger XZ-3Sstrain with producing cellulase includes 6∶2of wheat bran/corn cob ratio,4.0% ammonium sulfate,0.4%Twain-40,initial pH 4.0,28℃,96 hand 1∶1.5of medium material/water ratio.The activity of CMC and FPA enzyme of A.niger XZ-3Sstrain with producing cellulase can reach 273.56IU/g and 135.61IU/g under the optimal fermentation condition respectively.

Key concepts: Cellulase, Aspergillus niger, Bran, Fermentation, Chemistry, Solid-state fermentation, Food science, Cellulose

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