The effect of reformative Chinese herbs 921 compound combined with α-ketoglutarate on gut immunity in weaned rats

The present paper studied the effect of reformative Chinese herbs 921 compound combined with α-ketoglutarate(AKG)on the number of small intestine epidermic lymphoid Peyer's patch, area and the duodenal IgA secretion of 18-day's weaned rat with initial body weight 40.0±5 g. The rats were randomly divided into three groups: Control group (fed with conventional rat diet), Chinese herbs group (fed with conventional rat diet plus 4% Chinese herbs) and Chinese herbs combined AKG group (fed with conventional rat diet plus 4% Chinese herbs with 0.1% AKG). Each group has 10 rats. After 1 or 2 weeks feeding experiment respectively, the above-mentioned tests were conducted. The results showed: After 1 week feeding, the number of Peyer's patch in the whole intestine was16.4±1.23,14.2±0.63,15.1±0.68(P0.05) respectively.The duodenal IgA secretion was 0.844±0.020 g·L~(-1),0.921±0.039 g·L~(-1),1.236±0.131 g·L~(-1)(P0.01)respectively. After 2 weeks feeding, the number of Peyer's patch in the whole intestine was 15.6±0.50,16.4±0.94,15.6±0.99(P0.05)respectively, the area was 27.7±2.02 mm~2,44.8±4.34 mm~2,42.4±1.93 mm~2(P(0.01))in forepart respectively, 28.1±1.10 mm~2,40.5±5.68 mm~2,55.4±2.28 mm~2(P0.01)in middle part respectively, 37.6±4.57 mm~2,63.6±4.94 mm~2,59.0±5.35 mm~2(P0.01)in rear part respectively, the duodenal IgA secretion was 0.256±0.015 g·L~(-1),0.493±0.030 g·L~(-1),0.601±0.047 g·L~(-1)(P0.01)respectively. The concentration of duodenal IgA than in Chinese herbs plus AKG and in Chinese herbs group was significantly higher in control group. The study showed: Chinese herbs plus AKG can facilitate growth and development of gut through increasing the area of Peyer's patch in small intestine of weaned rats and mucosa immune function via increasing the IgA secretion.