STUDY ON EXTRACTION AND ABILITY TO SCAVENGE HYDROXYL RADICALS OF POLYSACCHARIDES FROM BARELY

To study the extraction technology and antioxidant activity of polysaccharides from barely.Barely was destarched and deproteined byα-Amylases and Protease,The factors including temperature,solid-liquid ratio and the extraction time on the yield of the polysaccharides were studied by orthogonal experimental design to se-lect the optimum extracting condition.The salicylic acid method was used to inspect the ability to scavenge hy-droxyl radicals of polysaccharides from barely.The results showed that optimal quantity of the two enzyme isα-Amylases 0.14 mL and neutral Protease 0.3 g.The optimum extracting conditions were:extraction temperature 100℃,the solid-liquid ratio 1:20 and the extraction time of 3 h.The yield and content of polysaccharides were 3.10%and 64.29%.The polysaccharides have good ability to scavenge hydroxyl radicals.The scavenging rate was 50%at the concentration of 5.88 g/L.The scavenging rate was 97.14%at the concentration of 20 g/L.