Improvement in quality control method for Compound Luxiancao Tablet

AIM To establish a simple and rapid method for the quality control of Compound Luxiancao Tablet(Sophorae flavescentis Radix,Balanophorae involucratae Herba,Smilacis glabrae Rhizoma,Aspongopus,Dioscoreae bulbifeerae Rhizoma and Trichosanthis Radix,etc.) to improve the current quality control.METHODS Sophorae flavescentis Radix,Balanophorae involucratae Herba,Smilacis glabrae Rhizoma,Aspongopus,Dioscoreae bulbifeerae Rhizoma and Trichosanthis Radix were identified by TLC on five thin-layer chromatographic plates.The contents of matrine and oxymatrine in Compound Luxiancao Tablet were determined by using a mixture of acetonitrile,methanol and 0.1% phosphoric(1.5∶ 4∶ 94.5) as the mobile phase by RP-HPLC.RESULTS The methodological study showed that a good linear correlation existed in the ranges of 0.043 5-1.087 5 μg(r=0.999 97) for matrine and 0.051 5-1.946 μg(r=0.999 99) for oxymatrine.The average recoveries of matrine and oxymatrine were 99.09% and 100.18%,respectively.RSDs were 1.39%(n=9) and 2.08%(n=9),respectively.The negative sample did not have interference on TLC identification.CONCLUSION The method in the improvement in quality standard of Fufang Luxiancao Tablet is simple,rapid and feasible.