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Quality standard of Xiaoyanxi Powder

Huang Yan

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Abstract

AIM To establish a quality standard of Xiaoyanxi Powder(Sophorae Flavescentic Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,etc.).METHODS Sophorae Flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were identified by TLC.Matrine and oxymatrine in Sophorae flavescentis Radix,and berberine in Phellodendri Chinensis Cortex were determined by HPLC.RESULTS Sophorae flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were detected by TLC.Matrine showed good linear relationship in the range of 0.200 8-2.008 μg(r = 0.999 21).Oxymatrine showed good linear relationship in the range of 0.208 4-2.084 μg(r = 0.998 85).Their average recovery was 98.68%(RSD was 1.408%).Berberine showed good linear relationship in the range of 0.204 8-1.024 μg(r = 0.998 85).The average recovery was 99.76%(RSD was 2.660%).CONCLUSION The method is simple and reproducible.It can be used to control the quality of Xiaoyanxi Powder.

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AIM To establish a quality standard of Xiaoyanxi Powder(Sophorae Flavescentic Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,etc.).METHODS Sophorae Flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were identified by TLC.Matrine and oxymatrine in Sophorae flavescentis Radix,and berberine in Phellodendri Chinensis Cortex were determined by HPLC.RESULTS Sophorae flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were detected by TLC.Matrine showed good linear relationship in the range of 0.200 8-2.008 μg(r = 0.999 21).Oxymatrine showed good linear relationship in the range of 0.208 4-2.084 μg(r = 0.998 85).Their average recovery was 98.68%(RSD was 1.408%).Berberine showed good linear relationship in the range of 0.204 8-1.024 μg(r = 0.998 85).The average recovery was 99.76%(RSD was 2.660%).CONCLUSION The method is simple and reproducible.It can be used to control the quality of Xiaoyanxi Powder.

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Available abstract

AIM To establish a quality standard of Xiaoyanxi Powder(Sophorae Flavescentic Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,etc.).METHODS Sophorae Flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were identified by TLC.Matrine and oxymatrine in Sophorae flavescentis Radix,and berberine in Phellodendri Chinensis Cortex were determined by HPLC.RESULTS Sophorae flavescentis Radix,Phellodendri Chinensis Cortex,Cnidii Fructus,Arnebiae Radix,Borneolum Syntheticum were detected by TLC.Matrine showed good linear relationship in the range of 0.200 8-2.008 μg(r = 0.999 21).Oxymatrine showed good linear relationship in the range of 0.208 4-2.084 μg(r = 0.998 85).Their average recovery was 98.68%(RSD was 1.408%).Berberine showed good linear relationship in the range of 0.204 8-1.024 μg(r = 0.998 85).The average recovery was 99.76%(RSD was 2.660%).CONCLUSION The method is simple and reproducible.It can be used to control the quality of Xiaoyanxi Powder.

Key concepts: Oxymatrine, Matrine, Radix (gastropod), Berberine, Traditional medicine, Quality standard, Linear relationship, Chromatography

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