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2003•South China Journal of Cardiovascular DiseasesRequires access

Effect of angiotensin II on expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells

Qiying Xie

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Abstract

Objectives To evaluate the effect of angiotensin Ⅱ on the protein and mRNA expression of monocyte chemoattractant protein 1(MCP 1) in cultured human umbilical vein endothelial cells(HUVECs) Methods The levels of protein and mRNA of MCP 1 were detected by ELISA and RT PCR respectively. Results Angiotensin Ⅱ distinctly increased the expression of MCP 1 in HUVECs; The highest expression was performed at 1×10 -7 mol/L angiotensin Ⅱ or at the 4th hour respectively; and losartan reduced the expression of MCP 1 remarkably. Conclusions Angiotensin Ⅱ can significantly increase the expression of MCP 1 in HUVECs,which is antagonized by losartan.

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Objectives To evaluate the effect of angiotensin Ⅱ on the protein and mRNA expression of monocyte chemoattractant protein 1(MCP 1) in cultured human umbilical vein endothelial cells(HUVECs) Methods The levels of protein and mRNA of MCP 1 were detected by ELISA and RT PCR respectively. Results Angiotensin Ⅱ distinctly increased the expression of MCP 1 in HUVECs; The highest expression was performed at 1×10 -7 mol/L angiotensin Ⅱ or at the 4th hour respectively; and losartan reduced the expression of MCP 1 remarkably. Conclusions Angiotensin Ⅱ can significantly increase the expression of MCP 1 in HUVECs,which is antagonized by losartan.

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Available abstract

Objectives To evaluate the effect of angiotensin Ⅱ on the protein and mRNA expression of monocyte chemoattractant protein 1(MCP 1) in cultured human umbilical vein endothelial cells(HUVECs) Methods The levels of protein and mRNA of MCP 1 were detected by ELISA and RT PCR respectively. Results Angiotensin Ⅱ distinctly increased the expression of MCP 1 in HUVECs; The highest expression was performed at 1×10 -7 mol/L angiotensin Ⅱ or at the 4th hour respectively; and losartan reduced the expression of MCP 1 remarkably. Conclusions Angiotensin Ⅱ can significantly increase the expression of MCP 1 in HUVECs,which is antagonized by losartan.

Key concepts: Umbilical vein, Losartan, Angiotensin II, Medicine, Monocyte, Renin–angiotensin system, Protein expression, Messenger RNA

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