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HPLC DETERMINAFION OF URSOLIC ACID IN HEDYOTIS DIFFUSA FROM DIFFERENT HABITAT

Qu Bi

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Abstract

Objective:To determine ursolic acid in Hedyotis diflusa Willd from difierent habitat and to provide a reference for quality control of raw materials.Methods:The analysis was performed by HPLC with Alltech RP-C18 column.The mobile phase consisted of methanol:water(90:10) at a flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm,and the column temperature was maintained at 30℃.Results: The linear range of ursolic acid was 10.916～152.824 μg/mL(r = 0.9997).The average recovery rate of ursolic acid was 98.48%,with RSD being 1.27%.Conclusion: The detemination method is simpIe,accurate and reproducible,and suitabIe for the quality control of Hedyotis diffusa Willd.

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What this paper is about

Objective:To determine ursolic acid in Hedyotis diflusa Willd from difierent habitat and to provide a reference for quality control of raw materials.Methods:The analysis was performed by HPLC with Alltech RP-C18 column.The mobile phase consisted of methanol:water(90:10) at a flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm,and the column temperature was maintained at 30℃.Results: The linear range of ursolic acid was 10.916～152.824 μg/mL(r = 0.9997).The average recovery rate of ursolic acid was 98.48%,with RSD being 1.27%.Conclusion: The detemination method is simpIe,accurate and reproducible,and suitabIe for the quality control of Hedyotis diffusa Willd.

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Available abstract

Objective:To determine ursolic acid in Hedyotis diflusa Willd from difierent habitat and to provide a reference for quality control of raw materials.Methods:The analysis was performed by HPLC with Alltech RP-C18 column.The mobile phase consisted of methanol:water(90:10) at a flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm,and the column temperature was maintained at 30℃.Results: The linear range of ursolic acid was 10.916～152.824 μg/mL(r = 0.9997).The average recovery rate of ursolic acid was 98.48%,with RSD being 1.27%.Conclusion: The detemination method is simpIe,accurate and reproducible,and suitabIe for the quality control of Hedyotis diffusa Willd.

Key concepts: Ursolic acid, High-performance liquid chromatography, Chromatography, Chemistry

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