2004•Zhongguo yaofangRequires access

Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Hedyotis Diffusa with HPLC

Zhao Shu-jin

Abstract

OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis diffusa.METHODS:The optimum HPLC conditions were as follows:Nucledure C 18 column(4.6mm×150mm);mobile phase:acetonitrile-potassium phosphate,pH=7.84(45∶55);detection wavelength:220nm.RESULTS:The average recoveries of oleanolic acid and ursolic acid were(96.79±2.42)%and(97.15±2.33)%respectively.CONCLUSION:The met_ hod could detect oleanolic acid and ursolic acid in Hedyotis diffusa simultaneously.It could be used in the quality control of the herb.

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Key concepts: Ursolic acid, Oleanolic acid, Chromatography, Chemistry, High-performance liquid chromatography, Traditional medicine, Medicine, Pathology