2012•Letters in BiotechnologyRequires access

Expression of PC-1 Gene During the Different Cell Cycle Phase of Prostate Carcinoma Cell Lines LNCaP and C4-2

Shi Qing

Abstract

Objective: To investigate the expression of PC-1 gene during cell cycle of prostate carcinoma cell lines.Methods: Two prostate carcinoma cell lines LNCaP and C4-2 were treated with 200 ng/mL nocodazole for 16h and entered into G2/M phase.Cells were harvested at different time points from 0 to 24 hours for flow cytometry analysis and Western blot.Results: Analysis of flow cytometry and Western blot showed that in both LNCaP and C4-2cell lines the expression of PC-1 was increased in G2/M phase.Conclusion: The expression of PC-1 is related with G2/M cell cycle,suggesting that PC-1 may play a role in cell cycle.

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Key concepts: LNCaP, Cell cycle, Flow cytometry, Nocodazole, Biology, Western blot, Cell culture, Molecular biology