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2011•Xiandai shengwu yixue jinzhanRequires access

Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative in COS-7 cells

Tuo Jin

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Abstract

Objective:To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine(PEI) derivative linking with glyoxal.Methods:Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.Cytotoxicity was detected by MTT method.Results:COS-7 cells transfection and cytotoxicity results suggested that the gene carrier(Polyimine-PEI) was less cytotoxicity than that of PEI25kDa,and expressed equal transfection efficiency with PEI25kDa and Lipofectamine 2000.Conclusion:Polyimine-PEI could be a highly efficient and less toxic gene carrier which was promising in gene therapy.

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What this paper is about

Objective:To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine(PEI) derivative linking with glyoxal.Methods:Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.Cytotoxicity was detected by MTT method.Results:COS-7 cells transfection and cytotoxicity results suggested that the gene carrier(Polyimine-PEI) was less cytotoxicity than that of PEI25kDa,and expressed equal transfection efficiency with PEI25kDa and Lipofectamine 2000.Conclusion:Polyimine-PEI could be a highly efficient and less toxic gene carrier which was promising in gene therapy.

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Available abstract

Objective:To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine(PEI) derivative linking with glyoxal.Methods:Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.Cytotoxicity was detected by MTT method.Results:COS-7 cells transfection and cytotoxicity results suggested that the gene carrier(Polyimine-PEI) was less cytotoxicity than that of PEI25kDa,and expressed equal transfection efficiency with PEI25kDa and Lipofectamine 2000.Conclusion:Polyimine-PEI could be a highly efficient and less toxic gene carrier which was promising in gene therapy.

Key concepts: Cytotoxicity, Transfection, Lipofectamine, Molecular biology, Reporter gene, Luciferase, Chemistry, Glyoxal

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