Effect of Paeonol on in Proliferation of Human Colorectal Cancer LoVo Cells and Its Mechanism

Objective:To investigate the effect of paeonol and aspirin on the proliferation of colorectal cancer line LoVo and discuss their possible mechanisms.Methods:LoVo cells were cultured in vitro.After treatment by paeonol and aspirin at different concentrations respectively at the end of24,48,72 and 96h,the cell survival was determined by the CKK-8method.Apoptosis was detected by flow cytometry.The concentration of intracellular calcium was investigated by laser confocal scanning microscope with calcium-fluorescent probes-Fluo-3/AM.Reverse transcriptionpolymerase chain reaction(RT-PCR)was used for mRNA analysis.Results:From the data of CKK-8,the cell proliferation of human colorectal cancer LoVo cells was inhibited by paeonol and aspirin in a dose-dependent and time-dependent manner.Flow cytometry assays showed that pae-onol and aspirin both significantly induced apoptosis in LoVo cells.After treated with paeonol for48 h,the apoptosis rate of Lovo cells was 13.5%,21.4% and 34.6% respectively,and after treated with aspirin,the apoptosis rate of Lovo cells was 14.8%,25.8%and 37.7%respectively,which showed an obvious concentration-effect relationship.The Ca2+relative fluorescence intensity of paeonol group was 41.36±4.62,57.51±3.83,and 69.43±3.76 respectively,and the Ca2+relative fluorescence intensity of aspirin group was 38.24±4.62,53.31±4.92,and 65.64±5.25 respectively.The concentration of intracellular calcium was significantly elevated by paeonol and aspirin.RT-PCR showed that paeonol and aspirin up-regulated RUNX3 in a dose-dependent manner in LoVo cells.Conclusion:Paeonol as well as aspirin can inhibit the proliferation of LoVo cells and induce apoptosis,and the mechanism of paeonol on apoptosis may be related to the upregulation of RUNX3 expression and the increase of concentration of intracellular calcium.The mechanism of paeonol and aspirin may be similar.