The effects of No.1 Kangnao liquid on the expresion of GFAP and the neuronal apoptosis after the focal cerebral ischemia-reperfusion in rats

Objective To observe the effect of No.1 Kangnao liquid on the expression of gelatinous fiber acidic protein (GFAP) and the apoptosis of neuronal cells after the focal cerebral ischemia-reperfusion(I/R) and to investigate the neuroprotection mechanism of No.1 Kangnao liquid.Methods The cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion(MCAO) for 2h,then followed by reperfusion at 1d,3d,7d.One hundred and eighty SD rats were randomly divided into five groups.The cerebral ischemia model was made in all the groups for observing the effects of No.1 Kangnao liquid on the expression of GFAP,the neurological function,cerebral infarction size and the pathomorphological changes of brain tissues.The TUNEL was used to measure the number of the neuronal apoptosis positive cells in brain of rats in the five groups.The expression of GFAP was detected by immunohistochemistry.Results As compared with those in the sham operation group,the numbers of neuronal apoptosis positive cells and GFAP positive cells were increased significantly in model group (P0.05).As compared with model group,No.1 Kangnao liquid reduced the score of neurological function as well as the cerebral infarction size,and relieved the degree of the pathomorphological change of cerebral tissues (P0.05).As compared with those in model group,the numbers of neuronal apoptosis positive cells and GFAP positive cells were decreased around the necrotic area in No.1 Kangnao liquid group B,however,the numbers of neuronal apoptosis positive cells and the expression of GFAP were increased in infarction central area in No.1 Kangnao liquid group.Conclusion No.1 Kangnao liquid can accommodate neuronal apoptosis and the expression of GFAP after focal cerebral I/R,as a result,which has neuroprotection effect.