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Performances of Five Methods for the Detection of Anti-dsDNA Antibody

Xin Song

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Abstract

To evaluate the performances of five methods for the detection of anti-dsDNA antibody.The serum anti-dsDNA antibody levels in 300 systemic lupus erythematosus(SLE) patients,300 disease control patients and 100 healthy peoples were detected by chemiluminescence immunoassay(CLIA),western blotting(WB),indirect immuno-fluorescence(IIF),enzyme-linked immuno-sorbent assay(ELISA) and colloidal gold colloid immunochromatography assay(CGCIA) respectively.The results showed that the highest sensitivity of assay was CLIA,followed by ELISA,CGCIA,WB and IIF.The highest specificity of Assay was IIF.There was no differences in the assay results between ELISA and CLIA,WB and CLIA(P0.05).The results measured by ELISA and CLIA matched very well with clinical diagnosis verification with high coincidence(Kappa=0.909).The results indicate that it may be the best way to measure anti-dsDNA antibody by ELISA and IIF simultaneously in clinical laboratory.

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What this paper is about

To evaluate the performances of five methods for the detection of anti-dsDNA antibody.The serum anti-dsDNA antibody levels in 300 systemic lupus erythematosus(SLE) patients,300 disease control patients and 100 healthy peoples were detected by chemiluminescence immunoassay(CLIA),western blotting(WB),indirect immuno-fluorescence(IIF),enzyme-linked immuno-sorbent assay(ELISA) and colloidal gold colloid immunochromatography assay(CGCIA) respectively.The results showed that the highest sensitivity of assay was CLIA,followed by ELISA,CGCIA,WB and IIF.The highest specificity of Assay was IIF.There was no differences in the assay results between ELISA and CLIA,WB and CLIA(P0.05).The results measured by ELISA and CLIA matched very well with clinical diagnosis verification with high coincidence(Kappa=0.909).The results indicate that it may be the best way to measure anti-dsDNA antibody by ELISA and IIF simultaneously in clinical laboratory.

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Available abstract

To evaluate the performances of five methods for the detection of anti-dsDNA antibody.The serum anti-dsDNA antibody levels in 300 systemic lupus erythematosus(SLE) patients,300 disease control patients and 100 healthy peoples were detected by chemiluminescence immunoassay(CLIA),western blotting(WB),indirect immuno-fluorescence(IIF),enzyme-linked immuno-sorbent assay(ELISA) and colloidal gold colloid immunochromatography assay(CGCIA) respectively.The results showed that the highest sensitivity of assay was CLIA,followed by ELISA,CGCIA,WB and IIF.The highest specificity of Assay was IIF.There was no differences in the assay results between ELISA and CLIA,WB and CLIA(P0.05).The results measured by ELISA and CLIA matched very well with clinical diagnosis verification with high coincidence(Kappa=0.909).The results indicate that it may be the best way to measure anti-dsDNA antibody by ELISA and IIF simultaneously in clinical laboratory.

Key concepts: IIf, Medicine, Antibody, Chemiluminescent immunoassay, Anti-dsDNA antibodies, Gold standard (test), Molecular biology, Immunoassay

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