Effects of different media composition on vitrification of Enstoma grandiflorun

The present study has been conducted to study the effect of different media and hormone composition on the differentiation of adventitious buds in Enstoma grandiflorun.The leaf pieces cultured in medium MS+6-BA 0.5 mg/L+IBA 0.2 mg / L(control medium) for 15 days were transferred to different media with different hormone composition(6-BA 0.2-0.5 mg / L;IBA 0.05-0.1 mg / L;NAA 0.01-0.2 mg / L;ZT 0.5-1.0 mg / L)for 30 days.The differentiated callus induced in control medium were transferred to MS medium every 3rd day and cultured for 30 days to obverse the differentiation of bud.The results showed that two media MS+6-BA 0.3 mg / L and MS+6-BA 0.4 mg / L +IBA 0.05mg / L were benefi-cial to the growth of adventitious bud.The total differentiated buds,total normal buds and average normal buds per leaf piece were found to be higher compared to control,while no significant difference was observed in rate of normal buds production.When the 6 days callus with buds were transferred to MS medium,the induction in total differentiated buds,total normal buds and average normal buds per leaf piece were found to be higher than that of the control,while no significant difference in rate of normal bud production was observed.Data revealed that the differentiation of buds induced from callus requires the exogenous hormone to reduce the vitrification.