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Taq DNA Polymerase

David H. Gelfand

Open publisher page 111 citations

Abstract

The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E. coli DNA Polymerase I, Klenow fragment (PolI-Kf).2,3 Since Taq DNA Polymerase can withstand repeated exposure to the high temperatures (94°–95°C)1 required for strand separation, the tedium and frequent rebellion resulting from having to add PolI-Kf after each cycle is minimized.

About this research paper

What this paper is about

The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E. coli DNA Polymerase I, Klenow fragment (PolI-Kf).2,3 Since Taq DNA Polymerase can withstand repeated exposure to the high temperatures (94°–95°C)1 required for strand separation, the tedium and frequent rebellion resulting from having to add PolI-Kf after each cycle is minimized.

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OpenAlex reports 111 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E. coli DNA Polymerase I, Klenow fragment (PolI-Kf).2,3 Since Taq DNA Polymerase can withstand repeated exposure to the high temperatures (94°–95°C)1 required for strand separation, the tedium and frequent rebellion resulting from having to add PolI-Kf after each cycle is minimized.

Key concepts: Klenow fragment, Thermus aquaticus, Taq polymerase, Hot start PCR, DNA polymerase, DNA polymerase I, Molecular biology, Polymerase

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